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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Optimized Production of L-phenylalanine by Fermentation Using Crude Glycerol) ผู้เขียน: ดร.อนุสิษฐ์ ธนะพิมพ์เมธา, รองศาสตราจารย์ ,  กล้า ธานีโต, เมธี ขำดวง, Kanoktip Packdibamrung, ดร.เพ็ญจิตร ศรีนพคุณ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractCrude glycerol, a by-product of the commercial production of biodiesel, was used directly as the carbon source to produce the essential amino acid L-phenylalanine in an optimized fermentation process involving the novel recombinant bacterium Escherichia coli BL21(DE3). In developing the optimal process, a Plackett-Burman experimental design was used to identify the components of the medium that most affected the production of the cell mass and L-phenylalanine. For the specified carbon source and with ammonium sulfate as the nitrogen source, the media components that were the most influential were: KH2PO4, K2HPO4, NaCl, MgCl2, CaCl2, CoCl2, CuSO4, Na2MoO4, ZnSO4 and yeast extract. A three-level and five-factor central composite experimental design was then used to identify the optimal concentrations of the most influential components of the medium and the optimal duration of the fermentation. Under the optimal combination of conditions for biomass production, a biomass concentration of 1.23 g/L was achieved in 32.43 h. Similarly, under the optimal conditions for production of L-phenylalanine, a metabolite concentration of 1.03 g/L was achieved in 30.29 h. A high correlation between the models’ predicted responses and the actual responses achieved in independent validation experiments confirmed the validity of the models. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Optimization of Medium Composition for L-phenylalanine Production from Glycerol using Response Surface Methodology) ผู้เขียน:เมธี ขำดวง, จรัญ ฉัตรมานพ, Kanoktip Packdibamrung, ดร.เพ็ญจิตร ศรีนพคุณ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractL-phenylalanine was produced by genetically modified bacterium, Escherichia coli BL21(DE3), using glycerol as an alternative carbon source. Response surface methodology (RSM) involving central composite design (CCD) was adopted to evaluate the amount of L-phenylalanine produced. In this work, the optimum concentrations were determined of the major nutrients in the fermentation medium, which included glycerol, (NH4)2SO4, MgCl2, K2HPO4, KH2PO4, yeast extract and thiamine-HCl. Analysis using biomass weight (gL-1) and amino acid production (gL-1), indicated that the optimum medium composition and concentration for the biomass production were: glycerol 10 gL-1, (NH4)2SO4 10 gL-1, MgCl2 0.98 gL-1, K2HPO4 2.94 gL-1, KH2PO4 2.94 gL-1, yeast extract 0.878 gL-1 and thiamine-HCL 0.0878 gL-1, with a maximum biomass weight of 5.0 gDCWL-1. In addition, the optimum medium composition for L-phenylalanine production was: glycerol 10 gL-1, (NH4)2SO4 100 gL-1, MgCl2 0.64 gL-1, K2HPO4 1.91 gL-1, KH2PO4 1.91 gL-1, yeast extract 0.823 gL-1 and thiamine-HCl 0.0823 gL-1. The highest L-phenylalanine weight at the optimum nutrient concentration was 6.2 gL-1. |
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Researcherดร. เพ็ญจิตร ศรีนพคุณ, ศาสตราจารย์ที่ทำงาน:ภาควิชาวิศวกรรมเคมี คณะวิศวกรรมศาสตร์ สาขาที่สนใจ:Sustainable energy and environmental engineering, Chemical and biochemical processes, Biofuels and biorefinery, Biodiesel and commercial production Resume |
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